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CRK12 and CYC9 interact in a yeast two hybrid assay. A: β-galactosidase assay for transcription of LacZLister 427 cells. Trypanosomes were being transfected with Just about every plasmid individually, then subjected to a next transfection with one of several other plasmids, with a different resistance gene, with all pairwise mixtures done.promoter